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Perpetual Taq DNA PolymeraseHOT START

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tiTaq DNA Polymerase HOT START
E2715-0S
5 u 0 EUR
+%20 KDV
0,00 TL
on Taq DNA Polymerase HOT START
E2713-0S
5 u 0 EUR
+%20 KDV
0,00 TL
tiOptiTaq DNA Polymerase HOT START
E2725-0S
5 u 0 EUR
+%20 KDV
0,00 TL
Perpetual Taq DNA PolymeraseHOT START
E2700-0S
5 u 0 EUR
+%20 KDV
0,00 TL
Perpetual Taq DNA PolymeraseHOT START
E2700-01
200 u 108,8 EUR
+%20 KDV
5.352,96 TL
tiTaq DNA Polymerase HOT START
E2715-04
500 U 110,4 EUR
+%20 KDV
5.431,68 TL
Perpetual Taq DNA Polymerase HOT START Kit
EK2700-01
200 u 120 EUR
+%20 KDV
5.904,00 TL
Perpetual Taq DNA Polymerase HOT START Kit
EK2700-01
1000 u 120 EUR
+%20 KDV
5.904,00 TL
on Taq DNA Polymerase HOT START
E2713-04
500 U 126,4 EUR
+%20 KDV
6.218,88 TL
tiOptiTaq DNA Polymerase
E2725-04
500 U 144 EUR
+%20 KDV
7.084,80 TL
tiTaq DNA Polymerase HOT START
E2715-02
1000 U 195,2 EUR
+%20 KDV
9.603,84 TL
on Taq DNA Polymerase HOT START
E2713-02
1000 U 224 EUR
+%20 KDV
11.020,80 TL
Perpetual Taq DNA PolymeraseHOT START
E2700-04
500 u 246,4 EUR
+%20 KDV
12.122,88 TL
tiOptiTaq DNA Polymerase
E2725-02
1000 U 256 EUR
+%20 KDV
12.595,20 TL
Perpetual Taq DNA Polymerase HOT START Kit
EK2700-04
500 u 274,4 EUR
+%20 KDV
13.500,48 TL
Perpetual Taq DNA PolymeraseHOT START
E2700-02
1000 u 417,6 EUR
+%20 KDV
20.545,92 TL
Perpetual Taq DNA PolymeraseHOT START
E2700-03
5000 u 1982,4 EUR
+%20 KDV
97.534,08 TL
Perpetual Taq DNA Polymerase HOT START Kit
EK2700-03
5000 u 2262,4 EUR
+%20 KDV
111.310,08 TL

 

Pre-complexed with specific anti-Taq monoclonal antibody, top quality thermophilic Taq DNA polymerase for automatic "hot start" PCR, resulting in greatly enhanced amplification specificity, sensitivity and yield.

Source: Thermus aquaticus

Description:

  • Ultrapure, recombinant Taq DNA Polymerase is reversibly complexed with anti-Taq monoclonal antibody that blocks replication activity of the enzyme at moderate temperatures.
  • Our carefully selected anti-Taq antibodies have high thermal stability, providing protection against non-specific primer extension from room temperature to 70°C.
  • The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94-95°C for two minutes.
  • Formation of complexes between Taq DNA Polymerase and an anti-Taq antibody forms a basis for automatic "hot start" PCR, which allows for the assembly of PCR reactions at room temperature.
  • High stability of the complexes allows for the enormous increase of PCR specificity, sensitivity and yield in comparison to the conventional PCR assembly method.
  • Automatic "hot start" PCR is a fast and convenient method when assembling multiple PCR reactions.
  • Both increased specificity and reduced mispriming improve multiplex PCR.
  • Eliminated risk of template cross-contamination and assured safe laboratory practice, due to removed necessity to open hot tubes.
  • Thermostable Taq DNA Polymerase replicates DNA at 74°C and exhibits a half-life of 40 min at 95°C (1,2).
  • Catalyzes the polymerization of nucleotides into duplex DNA in the 5'->3' direction in the presence of magnesium ions.
  • Contains the 5'->3' exonuclease activity.
  • Lacks the 3'->5' exonuclease activity.
  • Adds extra A at the 3' ends.
  • Perpetual Taq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.

Storage Buffer: 20 mM Tris-HCl (pH 8.0 at 22°C), 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 50% glycerol and stabilizers.

10 x Reaction Buffer:

10 x Pol Buffer A (optimization buffer without MgCl2): the buffer allows to optimize MgCl2concentration.

10 x Pol Buffer B (general application, up to 10 kb): the buffer contains 15 mM MgCland is optimized for use with 0.2 mM of each dNTP.

10 x Pol Buffer C (coloured): 10 x Pol Buffer B enriched with two gel tracking dyes and a gel loading reagent. The buffer enables direct loading of PCR products onto an agarose gel.

Quality Control: All preparations are assayed for contaminating endonuclease, 3-exonuclease, and non-specific single- and double-stranded DNase activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.

References:

  1. Chien, A., Edgar, D.B. and Trela, J.M. (1976) J. Bacteriol. 127, 1550.
  2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980) Biokhimiya 45, 644.

 

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