5x HOT FIREPol® EvaGreen®qPCR Supermix
Suitable for ROX-dependent and ROX-independent qPCR cyclers
For in vitro use only
Description:
HOT FIREPol® EvaGreen® qPCR Supermix is an optimised ready-to-use solution for real-time quantitative PCR assays, including EvaGreen® dye. It comprises all the components necessary to perform qPCR excluding the template and primers, to perform highly sensitive qPCR.
HOT FIREPol®DNA Polymerase is activated by a 12 min incubation step at 95°C. Hot-start mechanism prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Mix compatibility
Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™,Chromo4™, Opticon® 2 & MiniOpticon®
Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000
Eppendorf: Mastercycler®: ep realplex2 & ep
realplex4
Illumina: The Eco™
Roche: LightCycler® 480
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