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Human DNA Polymerase

Marka:EURx

Ürün Ölçü Fiyat TL Miktar
DNA Polymerase Beta (Human)
E1077-01
50 U 336 EUR
+%20 KDV
16.531,20 TL
DNA Polymerase Alpha (Human)
E1075-01
50 U 432 EUR
+%20 KDV
21.254,40 TL
DNA Polymerase Beta (Human)
E1077-02
200 U 1152 EUR
+%20 KDV
56.678,40 TL
DNA Polymerase Alpha (Human)
E1075-02
200 U 1472 EUR
+%20 KDV
72.422,40 TL

 

DNA Polymerase Beta (Human)

Description: 
simplest DNA polymerase known in both size and catalysis (1).
- a repair polymerase able to synthesize DNA beyond the end of gap or nick with simultaneous displacement of the non-replicated strand (2).
- fills gaps or nicks (3).

Unit definition: One unit is the amount of enzyme required to incorporate 1 nmole of total nucleotide into acid-insoluble form in 60 min. at 37°C.

1 x Reaction Buffer: 50 mM Tris-HCl (pH 8.7), 10 mM MgCl2, 0.4 mg/ml bovine serum albumin, 1.0 mM dithiothreitol, 100 mM KCl, 15% glycerol.

Storage Buffer: 20 mM Tris-HCl, pH 8.0, 1.0 mM dithiothreitol, 0.1 mM EDTA, 0.2 M NaCl and 50% (v/v) glycerol.

Assay conditions: 50 mM Tris-HCl, pH 8.7, 10 mM MgCl2, 0.4 mg/ml bovine serum albumin, 1.0 mM dithiothreitol, 100 mM KCl, 15% glycerol, 0.05 mM each dCTP, dGTP, dTTP, [α-32P]dATP and 10 µg activated DNA. Incubation is at 37°C for 15 min in a reaction volume of 50 µl.

Quality Control: All preparations are assayed for contaminating endonuclease and exonuclease activities.

Storage conditions: Store at -20°C.

References:

  1. Abbotts, J., SenGupta, D.N., Zmudzka, B., Widen, S.G., Notario, V., and Wilson, S.H. (1988) Biochemistry 27, No. 3, 901-909. 

  2.  Nowak, R., Kulik, J., and Siedlecki, J.A. (1987) Acta Biochim. Pol. 34, 205-215.

  3.     Wang, T.S-F., and Korn, D., (1980) Biochemistry 19, 1782-1790.

 

DNA Polymerase Alpha (Human)

Unit definition: One unit is the amount of enzyme required to incorporate 1 nanomole of total nucleotide into acid-insoluble form in 60 min. at 37°C.

Storage conditions: Store at -80°C for long-term, or -20°C for short-term storage.

1 x Reaction Buffer: 60 mM Tris-HCl (pH 8.0), 5.0 mM magnesium acetate, 0.3 mg/ml bovine serum albumin, 1.0 mM dithiothreitol, 0.1 mM spermine.

Storage Buffer: 20 mM Tris-HCl, pH 8.0, 0.25 mM EDTA, 50 mM NaCl, 1 mM ß-mercaptoethanol, 0.1% Triton X-100, and 50% (v/v) glycerol.

Assay conditions: 60 mM Tris-HCl, pH 8.0, 5.0 mM magnesium acetate, 0.3 mg/ml bovine serum albumin, 1.0 mM dithiothreitol, o.1 mM spermine, 0.05 mM each dCTP, dGTP, dTTP, dATP, (pH 7.0), [α-³H]dATP, and 20 µg activated calf thymus DNA. Incubation is at 37°C for 30 min. in a reaction volume of 50 µl.

Quality Control: The polymerase is tested for significant polymerase and primase activity. All preparations are assayed for contaminating endonuclease, 3’- and 5’-exonuclease, nonspecific RNase, and single- and double-stranded DNase activities.

References:

1. Podust, V.N., Lavrik, O.I., Nasheuer, H.-P., and Grosse, F. (1989) FEBS Letters 245, 14-16

 

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