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HOT TERMIPol DNA Polymerase

Ürün Ölçü Fiyat TL Miktar
HOT TERMIPol DNA Polymerase
01-06-KIT-0000S
50 U 0 EUR
+%20 KDV
0,00 TL
HOT TERMIPol DNA Polymerase
01-06-KIT-00500
500 U 198 EUR
+%20 KDV
9.741,60 TL
HOT TERMIPol DNA Polymerase
01-06-KIT-02000
2000 U 720 EUR
+%20 KDV
35.424,00 TL

Description: 

HOT TERMIPol® DNA Polymerase is a thermostable DNA polymerase suited for MALDI-TOF mass spectrometry and other primer extension platforms. The enzyme has 5’→3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs and labeled ddNTPs).

HOT TERMIPol® DNA polymerase is a chemically modified TERMIPol® DNA polymerase. At ambient temperatures it is inactive, having no polymerase activity. HOT TERMIPol® DNA polymerase is fully activated by a 8-12 minute incubation at 95ºC. This prevents extension of non-specifically annealed primers.

Applications:

- Primer extension

- MALDI-TOF mass spectrometry

- Mass-array

 

Concentration:

5 U/µl

 

Reagents Provided:

HOT TERMIPol® DNA Polymerase 

- 10x Reaction Buffer C 
  500 mM Tris-HCl pH 9.5 at 25ºC 

- 100 mM MgCl2

 

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.

 

Shipping and Storage Conditions:

Routine storage: -20ºC 
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT TERMIPol® DNA polymerase.

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