Welcome To BM Labosis

Sepet

Bst DNA Polymerase (Large Fragment, exo)

Marka:EURx

Ürün Ölçü Fiyat TL Miktar
Bst DNA Polymerase (Large Fragment, exo)
E1078-02
500 u 76,8 EUR
+%20 KDV
3.778,56 TL
Bst DNA Polymerase (Large Fragment, exo)
E1078-01
1600 u 94,032 EUR
+%20 KDV
4.626,37 TL


Bst DNA Polymerase (Large Fragment, exo -)

 

Source: Bacillus stearothermophilus

Large exonuclease free fragment of thermophilic Bst DNA Polymerase with strand displacement activity.

Description:

  • Bst DNA Polymerase is a moderately thermostable enzyme from Bacillus stearothermophilus.

  • Ultrapure, recombinant protein.

  • The enzyme replicates DNA optimally at 65°C.

  • Catalyzes the polymerization of nucleotides into duplex DNA in the 5´->3´ direction in the presence of magnesium ions.

  • Lacks the 5´->3´ exonuclease activity, while retaining the polymerase activity (1).

  • Broad activity range; can replace mezophilic polymerases as well as synthesize DNA at high temperatures. Thus it is suitable for amplification of difficult DNA templates, including repetitive sequences, GC-rich regions and problematic secondary structures (2,3).

  • Can be heat inactivated at temperatures above 80°C.

  • Active over wide range of reaction buffer conditions and magnesium ions concentrations.

  • Used in isothermal DNA sequencing at elevated temperatures.

  • Ideal for DNA synthesis reactions requiring strand displacement.

  • Exhibits thermophilic reverse transcriptase activity.

  • Used in isothermal nucleic acids amplification.

Unit Definition: One unit is the amount of enzyme required to incorporate 10 nmoles of total deoxyribonucleotide into acid-insoluble form in 30 min at 60°C.

Storage Conditions: Store at –20°C.

1 x Reaction Buffer: 50 mM Tris-HCl, (pH 8.9 w 20oC), 10 mM (NH4)2SO410 mM KCl, 2 mM MgSO40.1% Triton™X-100.

Storage Buffer: 20 mM potassium phosphate (pH 6.8), 1 mM dithiothreitol and 50% (v/v) glycerol.

Quality Control: All preparations are assayed for contaminating endonuclease, exonuclease and single- and double-stranded DNase activities.

References:

  1. Stenesh, J. and Roe, B.A. (1972) Biochim. Biophys. Acta. 272, 156-166.

  2. Hugh, G. and Griffin, M. (1994) PCR Technology, p.p.228-229.

  3. McClary, J. et al. (1991) J. DNA Sequencing and Mapping, p.p.173-180.

 

www.bmlabosis.com

Bu ürün BM Laboratuvar sistemleri tarafından ithal edilmektedir.

 

Product Size Price TL Quaintity