Vivid Violet DNA / RNA Co - Precipitate

Highly visible inert co-precipitant designed for routine use in DNA or RNA precipitations.

Description:

• Vivid Violet is a neutral carrier which enhances the recovery of nucleic acids when added prior to ethanol precipitation.
• Eliminates the need for low incubation temperature.
• Functions similarly to RNA or glycogen to improve recovery when precipitating dilute solutions of nucleic acids, but has the advantage of adding no biological material.
• Chemically synthesized; will not interfere in subsequent molecular biology manipulations.
• Results in a highly visible purple pellet after centrifugation of the precipitation mixture and thus eliminates loss of nucleic acid pellets during ethanol wash steps since the nucleic acids are readily seen.
• Avoids the necessity of being removed prior to next molecular biology step.
• Helps to determine when nucleic acids are thoroughly dissolved.
• Does not interfere with SDA, PCR, RT-PCR, restriction enzyme digestion, gel electrophoresis or fluorometric determination of nucleic acid content.
• Does not precipitate nucleic acids shorter than 20 base pairs, unincorporated nucleotides and short primers from PCR products can be removed.

Storage Conditions: Store at 4°C.

Stored In: Nuclease-free water.

Note 1: Sufficient for: 100 x 50 µl precipitations. Supplied at 10 mg/ml.

Note 2: Vivid Violet does absorb at A260 and therefore a correction of the absorbance reading is required.

Quality Control: All preparations are assayed for contaminating endonuclease, exonuclease, nonspecific RNase and single- and double-stranded DNase activities.