BM Labosis'e Hoşgeldiniz


Thermophilic Pyrophosphatase (Thermus aquaticus)


Ürün Ölçü Fiyat TL Miktar
Thermophilic Pyrophosphatase (Thermus aquaticus)
50 u 108,8 EUR
+%20 KDV
4.439,04 TL
Thermophilic Pyrophosphatase (Thermus aquaticus)
250 u 435,2 EUR
+%20 KDV
17.756,16 TL

Thermostable pyrophosphatase, hydrolyzing inorganic pyrophosphate into phosphate.


  • Hydrolyzes pyrophosphate, a common product of biosynthetic reactions (1).
  • Maintains the forward direction of reactions generating pyrophosphate.
  • Improves PCR amplification of problematic or long templates.
  • Used as an enzyme label for ELISA (2).

    Unit Definition: One unit will liberate 1 nmol of inorganic orthophosphate per 10 min at pH 7.5 at 65°C.

    Storage Conditions: store at -20°C.

    Storage Buffer: 20 mM Tris-HCl (pH 7.5 at 22°C), 50 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA and 50% (v/v) glycerol.

    Assay Conditions: 40 mM Tris-HCl (pH 7.5 at 22°C), 40 mM potassium acetate, 40 mM imidazole, 1 mM sodium pyrophosphate and 2.5 mM MgCl2. Incubation is at 65°C for 10 min in a reaction volume of 100 µl.

    Quality Control: All preparations are assayed for contaminating endonuclease, exonuclease and nonspecific RNase and single- and double-stranded DNase activities.

    1. Baykov, A. A., Kasho, V.N. and Avaeva, S.M. (1988) Anal. Biochem. 171, 271-276.2. Jerhoeven, J.A. (1986) J. Bacteriol. 168, 318-321.
    3. Richter, O. M. and Schafer, G. (1992) Eur. J. Biochem. 209, 343-349.

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