Source: Thermus aquaticus
Thermostable single-stranded specific DNA binding protein from Thermus aquaticus, suitable for high temperature DNA manipulations.
Description:
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Thermostable single-stranded specific DNA binding protein (1).
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Helix destabilizing protein (1).
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Reduces formation of problematic secondary DNA structures.
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Prevents degradation of ssDNA by nucleases.
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Ultrapure recombinant protein.
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Prevents inhibition of PCR by template DNA contaminants (2).
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Improves the efficiency of DNA amplification by Taq DNA Polymerase (3,4,5,6).
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Improves the specificity and selectivity of multiplex PCR (7).
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Aids PCR of difficult and GC-rich templates.
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Stabilizes single-stranded regions of DNA for site-specific mutagenesis.
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Aids completion of restriction enzyme digestion.
Storage Buffer: 10 mM Tris-HCl (pH 7.5 at 22°C), 50 mM NaCl, 5 mM β-mercaptoethanol, 0.05% Igepal, 0.1 mM EDTA and 50% (v/v) glycerol.
Quality Control: All preparations are assayed for contaminating endonuclease, 3'- and 5'-exonuclease activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.
References:
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Greipel, J. Urbanke, C. and Maass, G. (1989) in: Saenger, W., Heinemann, U. (Eds.) pp. 61-86.
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Kreader, C.A. (1996) Applied Environ. Micro. 62, 1102-1106.
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D�browski, S., Olszewski, M., Pi�tek, R. and Kur, J. (2002) Protein Expr. Purif. 26, 131-138.
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D�browski, S. and Kur, J. (1999) Protein Expr. Purif. 16, 96-102.
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Rapley, Mol. Biotech. 2 (1994) 295-298.
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Schwarz, K., Hansen-Hagge, T. and Bartram, C. (1989) Nucleic Acids Res. 18, 1079.
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Barski, P., Piechowicz, L., Galinski, J. and Kur, J. (1996) Mol. Cell Probes 10, 471-475.