SV40 Large Antigen (Unique!)

The major regulatory protein of SV40 virus, responsible for virion assembly, viral and cellular transcriptional regulation, viral DNA replication, alteration of the cell cycle; it also functions as molecular chaperone.

Note:This product is functionally warranted only when used in conjunction with our SV40 DNA Replication Assay Kit, Cat. No. E8050, containing control DNA, reaction buffer and HeLa extract.

Storage Conditions: Store at -20°C

Quality Control: All preparations are assayed for stimulation of DNA synthesis in a T antigen-dependent DNA replication assay (2). Typical preparations are greater than 90% pure as judged by SDS polyacrylamide gel electrophoresis.

References:

1. Evan, G. and Littlewood, T. (1998) Science 281,1317-1321.

2. Wold, M.S., Weinberg, D.H., Virshup, D.M., Li, J.J. and Kelly, T.J. (1989 ) J. Biol. Chem. 264, 2801-2809.

3. Thomas, D.C., Veaute, X., Kunkel, T.A. and Fuchs, R.P.P. (1994) Proc. Natl. Acad. Sci. 91, 7752-7756.

4. Boyer, J.C., Thomas, D.C., Maher, V.M., McCormick, J.J. and Kunkel, T.A. (1993) Cancer Res. 53, 3270-3275.

5. Bebenek, K., Thomas, D.C., Roberts, J.D., Eckstein, F. and Kunkel, T.A. (1993) Mol. Pharm. 43, 57-63.

6. Stillman, B. (1994) Cell 78, 725-728.

7. Fanning, E. (1992) J. Virol. 66, 1289-1293.

8. Dixon, R.A.F., and Nathans, D. (1985) J. Virol. 53, 1001-1004.