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Single-stranded DNA Binding Protein (E.coli)

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Ürün Ölçü Fiyat TL Miktar
Single-stranded DNA Binding Protein (E.coli)
E4200-02
 500 μg 288 EUR
+%20 KDV		 14.169,60 TL

Source: Escherichia coli

Description:

  • Single-stranded specific DNA binding protein (1).
  • Helix destabilizing protein (1).
  • Reduces formation of secondary DNA structures.
  • Prevents degradation of ssDNA by nucleases.
  • Ultrapure recombinant protein.
  • Prevents inhibition of PCR by template DNA contaminants (2).
  • Improves the efficiency of DNA amplification by Taq Polymerase (3,4,5,6).
  • Replaces Hot Start method during assembling PCR reaction (4,7).
  • Stabilizes single-stranded regions of DNA for site-specific mutagenesis.
  • Aids completion of restriction enzyme digestion.
  • Improves the efficiency of DNA synthesis by T4 DNA Polymerase (8).
  • Enhances the fidelity of modified T4 Polymerase (8).

Storage Buffer: 20 mM Tris-HCl (pH 7.8 at 22°C), 300 mM NaCl, 5 mM β-mercaptoethanol, 0.05% Igepal, 0.2 mM EDTA and 50% (v/v) glycerol.

Quality Control: All preparations are assayed for contaminating endonuclease, 3'- and 5'-exonuclease activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.

References:

  1. Greipel, J. Urbanke, C. and Maass, G. (1989) in: Saenger, W., Heinemann, U. (Eds.) pp. 61-86.
  2. Kreader, C.A. (1996) Applied Environ. Micro. 62, 1102-1106.
  3. D�browski, S., Olszewski, M., Pi�tek, R. and Kur, J. (2002) Protein Expr. Purif. 26, 131-138.
  4. D�browski, S. and Kur, J. (1999) Protein Expr. Purif. 16, 96-102.
  5. Rapley, Mol. Biotech. 2 (1994) 295-298.
  6. Schwarz, K., Hansen-Hagge, T. and Bartram, C. (1989) Nucleic Acids Res. 18, 1079.
  7. Barski, P., Piechowicz, L., Galinski, J. and Kur, J. (1996) Mol. Cell Probes 10, 471-475.
  8. Sandhu, D.K. and Keohavong, P. (1994) Gene 144, 53-58.
Ürün Ölçü Fiyat TL Miktar

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