Ribonuclease inhibitor suitable for use in enzymatic reactions.
Description:
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More potent than competing human placental RNase inhibitors.
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RNase Free.
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Effective over broad pH range from pH 5.5 to 8.5.
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Active over temperature range from 37°C to 70°C.
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Does not interfere with SP6, T7 or T3 RNA polymerase, AMV or M-MLV reverse transcriptase or Taq DNA polymerase.
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Increases the time RNA can be safely stored.
Unit Definition: One unit is the amount of ribonuclease inhibitor required to inhibit the activity of 5 ng of Ribonuclease A at 25°C (by 50%). Ribonuclease inhibitor activity is determined by the inhibition of RNase A hydrolysis of cyclic 2',3'-cytidine monophosphate in a spectrophotometer recording assay.
Storage Conditions: Store at -20°C.
Storage Buffer: 20 mM HEPES-KOH (pH 7.5), 100 mM KCl, 0.1 mM EDTA, 2 mM dithiothreitol and 50% (v/v) glycerol.
Assay Conditions: 0.1 M Tris-acetate (pH 6.5 at 22°C), 1 mM EDTA, 1 mM cyclic 2',3'-cytidine monophosphate. Reaction volume 1 ml.
Quality Control: All preparations are tested for contaminating endonuclease and exonuclease and nonspecific RNase and single- and double-stranded DNase activities.