PerfectStartTM Green qPCR SuperMix is a ready-to-use qPCR cocktail. It contains a PerfectStartTM Taq DNA Polymerase, optimized dual-cation buffer, SYBR Green I, dNTPs, PCR enhancer and PCR stabilizer. PerfectStartTM Taq DNA polymerase is a hot-start Taq DNA polymerase containing Taq DNA polymerase and three kinds of monoclonal antibodies, effectively blocking DNA polymerase activity and preventing non-specific amplification at low temperature. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers, passive reference dye (optional) and nuclease-free water.
• PerfectStartTM Taq DNA Polymerase enables high specificity, high sensitivity, high amplification efficiency.
• Dual-cation buffer enhances specificity and reduces primer-dimer formation.
• Passive reference dyes (to normalize tube-to-tube differences due to pipetting errors) are provided for different qPCR instruments.
Storage
at -20°C in dark for two years
Shipping
Dry ice (-70 ℃)
| Component |
AQ601-01 |
AQ601-02 |
AQ601-03 |
AQ601-04 |
| 2×PerfectStartTM Green qPCR SuperMix |
1 ml |
5×1 ml |
15×1 ml |
25×1 ml |
| Passive Reference Dye (50×) |
40 μl |
200 μl |
600 μl |
1 ml |
| Nuclease-free Water |
1 ml |
5 ml |
3×5 ml |
5×5 ml |