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Ovation® WGA FFPE System
The Ovation® WGA FFPE System provides a fast and simple method for preparing amplified DNA (SPIA®) from genomic DNA prepared from Formalin Fixed Paraffin Embedded (FFPE) tissue. The resulting amplified DNA may be used in a variety of downstream applications, including array CGH (aCGH) and sequencing library construction (requires the Encore™ ds-DNA Module). Because of the simplicity and robustness of the procedure, accurate and quantitative results are obtained with minimal dropouts and excellent coverage.
The Ovation WGA FFPE System is powered by SPIA® technology, a rapid, simple and robust nucleic acid amplification process developed by NuGEN. Starting with 100 ng of genomic DNA from FFPE tissue, microgram quantities of amplified SPIA product can be prepared in approximately six hours.
The Ovation WGA FFPE System (Part No. 6200) provides optimized reagent mixes to process 8 FFPE DNA samples for downstream labeling and hybridization on aCGH arrays, or construction of libraries using in next generation sequencing using the Encore ds-DNA Module.
With NuGEN's new Ovation WGA FFPE System you can survey the entire genome for structural changes with aCGH, explore detailed sequence variations with DNA sequencing, or use these methods together with the same starting DNA for a more complete and accurate understanding of genomic diversity.
Robust aCGH Performance Metrics from 100ng gDNA: Genomic analysis by aCGH has been a broadly used, high resolution tool to assess genomic architecture and to detect genomic changes relative to a reference control. The utility of this technique is often compromised with FFPE samples due to the high degree of DNA degradation and cross-linking caused by the fixation process. Performance metrics from aCGH experiments using our Ovation WGA FFPE System are summarized in Table 1.