Ovation® Whole Blood Solution
The Ovation® Whole Blood Solution is comprised of optimized products and user guides enabling gene expression analysis of whole blood RNA without the need for Globin reduction procedures. There is an amplification component, an optimized Whole Blood amplification reagent and a fragmentation and labeling module:
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Ovation® RNA Amplification System V2 (Cat.# 3100)
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Ovation® WB Reagent (Cat.# 1300)
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Encore™ Biotin Module (Cat.# 4200)
These components provide a fast, simple, automation-friendly process that starts with 20-50 ng of whole blood total RNA and yields up to 12 micrograms of ss-cDNA ready for qPCR analysis or cDNA storage. The cDNA can be used in fragmentation and labeling for target preparation and analysis with Affymetrix GeneChip® arrays. This solution enables analysis of nanograms of whole blood total RNA on GeneChip® arrays in one day. The Ovation® WB Reagent provides optimized reagent mixes and a protocol to process 12 or 60 RNA samples (cat.# 1300-12, 1300-60, 1300-A01 [automation]).
Ovation™ Whole Blood RNA amplification yields high array reproducibility: Duplicate samples were prepared from 20 ng of human whole blood RNA using the Ovation™ Whole Blood Solution, according to the product user guides. RNA samples were isolated using the Agencourt RNAdvance™ Blood kit on the Beckman ArrayPlex. Targets were hybridized to Affymetrix GeneChip® U133_plus_2.0 arrays. Results of linear regression analysis show a strong signal correlation of R = 0.992, with 58% present calls, demonstrating very high reproducibility across transcript abundance with only 20 ng of input total whole blood RNA.