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Ovation® RNA-Seq FFPE System


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Ovation® RNA-Seq FFPE System
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Ovation RNA-Seq FFPE System

The Ovation® RNA-Seq FFPE System provides a fast and simple method for preparing amplified cDNA from FFPE-derived total RNA for RNA-Seq applications (transcriptome sequencing). Amplification is initiated at the 3' end as well as randomly throughout the transcriptome in the sample, making the Ovation RNA-Seq FFPE System ideal for amplification of the severely degraded and chemically modified RNA typically obtained from FFPE samples. This approach is ideal for processing samples for RNA-Seq on Next Generation Sequencing (NGS) systems, as reads are distributed across the transcript. The Ovation RNA-Seq FFPE System is optimized for the generation of double-stranded cDNA suitable for sequencing library construction for use with a variety of NGS platforms.

The Ovation RNA-Seq FFPE System is powered by Ribo-SPIA® technology, a rapid, simple and sensitive RNA amplification process developed by NuGEN. Using Ribo-SPIA technology and starting with as little as 100 ng FFPE RNA, microgram quantities of cDNA can be prepared in approximately six hours.

The Ovation RNA-Seq FFPE System (Part No. 7150) provides optimized reagent mixes and a protocol to process eight RNA samples.

Principal Components Analysis Plot of Colon Tumor/Normal FFPE/Fresh Frozen Quad Set: Normal adjacent tissue (NAT) samples are in red, tumor samples are in blue. FFPE samples are represented as spheres and Fresh Frozen samples as boxes. Analysis was performed using the Partek Genomics Suite.

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