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Ovation® Prokaryotic RNA-Seq System

Marka:Nugen

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Ovation® Prokaryotic RNA-Seq System
9030-08
8 Reak. 0 USD
+%8 KDV
0,00 TL

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Ovation® Prokaryotic RNA-Seq System

The Ovation® Prokaryotic RNA-Seq System provides a method for the analysis of microbial species and microbiome samples by enabling whole transcriptome profiling. The core technology used in this product enriches for mRNA in NGS libraries, and can be applied to transcriptomes extracted from pure and mixed microbial cultures in an easy end-to-end workflow, from total RNA to sequencing. The first and second strand cDNA synthesis are carried out using proprietarily designed primers to create double-stranded cDNA . The resulting cDNA is compatible with NuGEN’s Ovation Ultralow Library Systems as well as other library workflows using double-stranded cDNA as input for the creation of sequencing libraries.

The Ovation Prokaryotic RNA-Seq System provides reproducible transcriptome profiles across all prokaryotic species in the classically defined Kingdoms of both Eubacteria and Archaebacteria. To date, we have validated the system’s performance across six bacterial species that cover a broad range of G:C base composition.

During construction of NGS transcriptome libraries, the first and second strand cDNA synthesis is performed in about 3.0 hrs with selective priming to enrich non-rRNA transcripts from bacterial and archaeal total RNA inputs. Sequencing libraries with optional use of multiplexing is performed in about 4.0 hrs with the Ovation Ultralow Library Systems or other suitable library construction methods.


Construction of NGS transcriptome libraries. 1st and 2nd strand cDNA synthesis with selective priming to enrich non-rRNA transcripts from bacterial and archaeal total RNA inputs. Following end repair, the double strand cDNA is compatible with NuGEN's Ovation Ultralow Library Systems that allow easy sample multiplexing within a single-end, transcriptome sequencing format.

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