MboI
(Moraxella bovis)
5’-G A T C-3’
3’-C T A G-5’
Reaction Temperature: 37°C
Prototype: MboI
Inactivation Temperature (20 min): 65°C
Reaction Buffer:
1x Medium Buffer: 10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 50 mM NaCl, 100 µg/ml bovine serum albumin.
Note: blocked by Dam methylation
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of unmethylated lambda DNA in 1 hr in a total reaction volume of 50 µl.
Assay Conditions: 10 mM Tris-HCl (pH 7.5 at 37°C), 50 mM NaCl, 10 mM MgCl2, 100 µg/ml bovine serum albumin and 1 µg of unmethylated lambda DNA. Incubation is at 37°C for 1 hr in a reaction volume of 50 µl.
Storage Buffer: 10 mM Tris-HCl (pH 7.5 at 22°C), 50 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml bovine serum albumin, 0.01% Triton™X-100 and 50% (v/v) glycerol.
Storage Conditions: Store at –20°C.
Quality Control:
Non-specific Endonuclease: Incubation of 10 units of MboI with 1 µg of unmethylated lambda DNA at 37°C for 16 hrs (a 160-fold over-digestion) resulted in the same sharp characteristic banding pattern as the standard assay reaction, as determined by agarose gel electrophoresis.
3'-Exonuclease: 5, 10 and 20 units of MboI and 0.13 µg of 3'-ends of lambda/TaqI fragments (3'-labeled with T4 DNA Polymerase and [a-³³P]dGTP and [a-³³P]dCTP), incubated for 1 hr at 37°C resulted in 0.11 slope of %-end label released per unit of enzyme. Reaction volume 10 µl.
5'-Exonuclease/5'-Phosphatase: Incubation of 5, 10 and 20 units of MboI with 0.05 µg of 5'-ends of [5'-³³P]lambda/HaeIII fragments for 1 hr at 37°C resulted in 0.01 slope of %-end label released per unit of enzyme.