GeneMATRIX Tissue & Bacterial DNA Purification Kit is designed for rapid purification of total DNA (genomic, mitochondrial) from a variety of tissues, biological liquids, cultured cells and bacteria. Purified DNA is free of contaminants, such as: RNA, proteins, lipids, dyes, detergents, organic inhibitors of enzymatic reactions, buffers, salts, divalent cations, among others.
Sample is proteolitycally lysed in the presence of special buffer, aiding tissue and cells desintegration. Further, Proteinase K digests cellular proteins, including stripping-off DNA of all bound proteins, among them nucleases. Optimized buffer and ethanol are added to provide selective conditions for DNA binding during brief centrifugation, while contaminants pass through the GeneMATRIX resin in the spin-column. Traces of contaminants remaining on the resin are efficiently removed in two wash steps. High-quality cellular DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE or water. Isolated DNA is ready for downstream applications without the need for ethanol precipitation.