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Color Taq DNA Polymerase

Marka:EURx

Ürün Ölçü Fiyat TL Miktar
Color Taq DNA Polymerase
E2510-01
200 u 42,4 EUR
+%20 KDV
2.086,08 TL
Color Taq DNA Polymerase Kit
EK2510-01
200 u 53,6 EUR
+%20 KDV
2.637,12 TL
Color Taq DNA Polymerase
E2510-04
500 u 92,8 EUR
+%20 KDV
4.565,76 TL
Color Taq DNA Polymerase Kit
EK2510-04
500 u 120,8 EUR
+%20 KDV
5.943,36 TL
Color Taq DNA Polymerase
E2510-02
1000 u 161,6 EUR
+%20 KDV
7.950,72 TL
Color Taq DNA Polymerase Kit
EK2510-02
1000 u 217,6 EUR
+%20 KDV
10.705,92 TL
Color Taq DNA Polymerase
E2510-03
5000 u 800 EUR
+%20 KDV
39.360,00 TL
Color Taq DNA Polymerase Kit
EK2510-03
5000 u 1080 EUR
+%20 KDV
53.136,00 TL

 

Stable thermophilic DNA polymerase, suitable for applications requiring high temperature synthesis of DNA.

The enzyme is supplemented with two inert gel tracking dyes.

Description:

  • Taq DNA Polymerase is a thermostable enzyme of approximately 94 kDa from Thermus aquaticus.
  • Ultrapure, recombinant protein.
  • The enzyme replicates DNA at 74°C and exhibits a half-life of 40 min at 95°C (1,2).
  • Catalyzes the polymerization of nucleotides into duplex DNA in the 5´->3´ direction in the presence of magnesium ions.
  • Maintains the 5´->3´ exonuclease activity.
  • Lacks the 3´->5´ exonuclease activity.
  • Adds extra A at the 3' ends.
  • Color Taq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.
  • Use of Color Taq DNA Polymerase offers several advantages:
    • visualizes the addition of the polymerase to the reaction,
    • confirms complete mixing,
    • enables direct loading of PCR products onto an agarose gel without addition of a gel loading buffer,
    • the added dyes allow to track electrophoresis progress,
    • do not affect PCR performance,
    • do not interfere with most downstream applications (exeption: the polymerase is not recommended for any downstream application using absorbance or fluorescence excitation). 

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 min at 74°C. The reaction conditions are: 50 mM Tris-HCl (pH 9.0 at 25°C), 50 mM NaCl, 5 mM MgCl2, 200 μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10 μg activated calf thymus DNA and 0.1 mg/ml BSA in a final volume of 50 μl.

Storage Conditions: Store at -20°C.

Storage Buffer: 20 mM Tris-HCl (pH 8.0 at 22°C), 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 50% glycerol and stabilizers.

10 x Reaction Buffer:

10 x Pol Buffer A (optimization buffer without MgCl2):  the buffer allows to optimize MgCl2concentration.

10 x Pol Buffer B (general application, up to 10 kb): the buffer contains 15 mM MgCland is optimized for use with 0.2 mM of each dNTP.

Quality Control: All preparations are assayed for contaminating endonuclease, 3'-exonuclease, and nonspecific single- and double-stranded DNase activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.

References:

  1. Chien, A., Edgar, D.B. and Trela, J.M. (1976) J. Bacteriol. 127, 1550.
    • Kaledin, A.S., Sliusarenko, A.G. i Gorodetskii, S.I. (1980) Biokhimiya 45, 644.
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