DNA ladder with 0.25-1 kb increments for sizing medium-to-large DNA fragments.
Description:
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Ideal for sizing linear double-stranded DNA fragments from 0.5 to 10.0 kb.
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Contains 13 bands with fragments of the following sizes: 0.25, 0.5, 0.75, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0, 5.0, 6.0, 8.0, and 10.0 kb.
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Bands at 1.0 and 3.0 kb are three times brighter for easy reference on agarose gels.
5 µl load would load 500 ng DNA:
10 kb 60 ng,
8 kb 50 ng,
6 kb 25 ng,
5 kb 15 ng,
4 kb 25 ng,
3 kb 60 ng,
2.5 kb 30 ng,
2 kb 35 ng,
1.5 kb 20 ng,
1 kb 80 ng,
0.75 kb 45 ng,
0.50 kb 25 ng,
0.25 kb 30 ng.
Storage Buffer: 10 mM Tris-HCl (pH 8.0 at 22°C), 1 mM EDTA, dye.
Loading: The recommended amount of size marker to load on a gel is 0.2-1.0 µg per lane depending on a gel type and size of well.
Concentration: The Perfect Plus™1 kb DNA Ladder is supplied at 500 µg/ml in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA.
Storage Conditions: Store at -20°C