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XbaI, (XbaI)

Marka:EURx

Ürün Ölçü Fiyat TL Miktar
XbaI
E2430-01
3 000 u 57,6 EUR
+%20 KDV
2.833,92 TL
XbaI
E2430-02
15 000 u 228,8 EUR
+%20 KDV
11.256,96 TL

XbaI

(Xanthomonas badrii)

5’-T C T A G A-3’
3’-A G A T C T-5’

Reaction Temperature: 37°C

Prototype: XbaI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x Medium Buffer: 10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 1 mM dithiothreitol, 50 mM NaCl, 100 µg/ml bovine serum albumin.

Note: blocked by overlapping Dam methylation.

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Ad-2 DNA in 1 hr in a total reaction volume of 50 µl.

Assay Conditions: 10 mM Tris-HCl (pH 7.5 at 37°C), 50 mM NaCl, 10 mM MgCl2, 1 mM dithiothreitol and 1 µg of Ad-2 DNA . Incubation is at 37°C for 1 hr in a reaction volume of 50 µl.

Storage Buffer: 10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 100 µg/ml bovine serum albumin and 50% (v/v) glycerol.

Storage Conditions: Store at –20°C.

Quality Control:

Non-specific Endonuclease: Incubation of 10 units of XbaI with 1 µg of Ad-2 DNA at 37°C for 16 hrs (a 160-fold over-digestion) resulted in the same sharp characteristic banding pattern as the standard assay reaction, as determined by agarose gel electrophoresis.

3'-Exonuclease: 5, 10 and 20 units of XbaI and 0.11 µg (0.80 pmol of 3'-ends) of lambda/TaqI fragments (3'-labeled with T4 DNA Polymerase and [³H]dGTP and [³H]dCTP), incubated for 1 hr at 37°C resulted in 0.01-slope of %-end label released per unit of enzyme. Reaction volume 10 µl.

5'-Exonuclease/5'-Phosphatase: Incubation of 5, 10 and 20 units of XbaI with 0.02 µg (0.15 pmol of 5'-ends) of [5'-³²P]lambda /HaeIII fragments for 1 hr at 37°C resulted in 0.02-slope of %-end label released per unit of enzyme. Reaction volume 10 µl.

 

 

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