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S1 Nuclease

Marka:EURx

Ürün Ölçü Fiyat TL Miktar
S1 Nuclease
E1335-01
10 000 u 57,6 EUR
+%20 KDV
2.833,92 TL
S1 Nuclease
E1335-02
50 000 u 225,6 EUR
+%20 KDV
11.099,52 TL

Source: Aspergillus oryzae

Non-specific endonuclease, active primarily on single-stranded DNA and RNA.

  • Catalyzes degradation of single-stranded nucleic acids into oligonucleotides and 5’-mononucleotides (1).

  • Cleaves both single-stranded DNA and RNA, with stronger DNAse acitivity.

  • Double-stranded DNA, double-stranded RNA and DNA-RNA hybrids are resistant to degradation at moderate enzyme concentration.

  • Capable of cleavage of double-stranded nucleic acids at nicks, mismatches and small gaps (2).

  • Relaxes/linearizes supercoiled plasmids.

  • Removes protruding single-stranded ends.

  • Used for S1 mapping of nucleic acids.

  • Requires Zn2+ ions for activity.

  • The enzyme is active up to 65°C.

Storage Buffer: 20 mM Tris-HCl (pH 7.5 at 22°C), 50 mM NaCl, 0.1 mM ZnCl2 and 50% (v/v) glycerol.

Assay Conditions: 30 mM sodium acetate (pH 4.6), 1 mM zinc acetate, 50 mM NaCl, 0.5 mg/ml of activated DNA, 5% glycerol. Incubation is at 37°C for 10 min in a reaction volume of 0.5 ml.

Quality Control: All preparations are assayed for contaminating exonuclease and double-stranded DNase activities.

References:

  1. Berg, A.J. et al. (1977) Cell 12, 721.

  2. Sambrook, J. et al. (1989) Molecular cloning: A laboratory Manual, second edition, pp. 5.78-5.79, Cold Spring Harbor, New York.

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