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Rec A Protein (E.coli)

Marka:EURx

Ürün Ölçü Fiyat TL Miktar
Rec A Protein (E.coli)
E1370-01
 0.2 mg 99,2 EUR
+%20 KDV		 4.880,64 TL
Rec A Protein (E.coli)
E1370-02
 1 mg 392 EUR
+%20 KDV		 19.286,40 TL

Bacterial recombination protein that enhances interaction and exchange of homologous DNA strands.

Description:

  • A 37,8 kDa protein encoded by the recA gene of E. coli.
  • Regulates repair mechanisms and repairs DNA (1, 2).
  • Involved in DNA renaturation and rare cleavage of genomic DNA.
  • Binds cooperatively and stechiometrically to single-stranded DNA.
  • Ultrapure recombinant protein.
  • In presence of ATP-g-S, locates and pairs a single-stranded DNA sequence to its homologous double-stranded DNA.
  • Cleaves large fragments of DNA (RARE-Rec A Assisted Restriction Endonuclease) (3).
  • Enhances contrast of electron micrography when DNA is coated with Rec A Protein (4).

    Storage Buffer: 20 mM Tris-HCl (pH 7.5 at 22°C), 1 mM dithiothreitol, 0.1 mM EDTA and 50% (v/v) glycerol.

    Storage Conditions: Store at -20°C.

    Quality Control: All preparations are assayed for endonuclease and nonspecific single- and double-stranded DNase activities. Typically preparations are greater than 95% pure as judged by SDS polyacrylamide gel electrophoresis.

    References:

    1. Clark, A.J. and Margulies, A.D. (1965) Proc. Natl. Acad. Sci. U.S.A. 53, 451-459.
    2. Radding, C.M. (1978) Annu. Rev. Biochem. 47, 847-880.
    3. Koob, M., Burkiewicz, A., Kur, J. and Szybalski, W. (1992) Nucleic Acids Res. 20, 5831-5836.
    4. Krasnow, M. A. et al. (1983) Nature 304, 559. contrast of electron micrography when DNA is coated with Rec A Protein (4).
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