Ovation® 3'-DGE System
The Ovation 3'-DGE System provides accurate expression analysis (tag profiling) suitable for quantification of any poly(A)+ transcripts, without the need for prior sequence knowledge. It employs a simple, fast and automatable workflow, powered by the Ribo-SPIA® Single Primer Isothermal Amplification technology and is compatible with all leading NGS platforms.
The input for the Ovation 3'-DGE System is flexible. You can choose one of the two options that best fits with your experimental design:
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10 ng – 100 ng of purified total RNA
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Direct cell lysate of 50 – 10,000 cells
The protocol for the Ovation 3'-DGE System takes only six hours to complete and generates micrograms of double-stranded cDNA, focusing on the most 3' 150 – 500 bases proximal to the poly(A) tail of the transcripts. The cDNA is suitable as input for any NGS library preparation kit, such as Encore® Rapid or Ovation Ultralow Library Systems.
DGE libraries can be produced in nine hours, greatly simplifying the entire sample preparation procedure. The combination of the Ovation 3'-DGE System and NuGEN's NGS Library Systems provides a complete, streamlined and easily automatable sample preparation workflow, enabling NGS-based expression profiling approaching the protocol simplicity and sample throughput of microarrays.
Figure 1. Simple and Fast Ovation 3'-DGE Workflow for Integrated NGS Analysis
Figure 2. Highly Concordant Differential Expression Results Obtained Comparing DGE and qPCR
