Ovation® Universal RNA-Seq System
The Ovation Universal RNA-Seq System provides an end-to-end solution for strand-specific RNA-Seq library construction using nanogram amounts of total RNA obtained from a broad range of tissues or cell lines. The integrated workflow (Figure 1) from input of total RNA to fully constructed library is highly reproducible, and can be completed in approximately 7 hours. Insert Dependent Adaptor Cleavage (InDA-C) technology enables targeted depletion of unwanted transcripts using a customizable process. As shown in Table 1, InDA-C technology can be applied to essentially any transcript type to reduce uninformative sequencing reads for more efficient use of sequencing resources. NuGEN provides a no cost design service to assist researchers in choosing InDA-C primers to target transcripts for depletion. Please contact [email protected] for further information.
The Ovation Universal RNA-Seq System (Part No. 0343-32) provides 16 unique barcoded adaptors to enable multiplex sequencing to further optimize efficiencies and cost savings in Transcriptome analysis. This kit provides all reagents necessary for RNA-Seq library construction using researcher supplied InDA-C primers. An example of how InDA-C technology can be used to deplete mouse rRNA sequencing reads is shown in Table 2.
The Ovation Universal RNA-Seq System has been designed for strand-specific expression analysis by incorporation of a nucleotide analogue during the second strand cDNA synthesis, and subsequent ligation, to a pair of double-stranded library adaptors also containing the same analogue in one strand. After ligation, the cDNA strand and adaptor containing the analogue are selectively removed (Strand Selection), leaving only one cDNA strand with both adaptor sequences attached. This product is then converted into a sequence-ready library by PCR amplification (see Figure 1).
Figure 1:
Table 1: Examples of targeted transcript depletion using InDA-C technology - Click to enlarge
Table 2: Targeted depletion of rRNA in mouse - Click to enlarge
