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NheI, (NheI)

Marka:EURx

Ürün Ölçü Fiyat TL Miktar
NheI
E2294-01
500 u 40 EUR
+%20 KDV
1.968,00 TL
NheI
E2294-02
2 500 u 160 EUR
+%20 KDV
7.872,00 TL

(Neisseria mucosa heidelbergensis)

5’-G C T A G C-3’
3’-C G A T C G-5’

Recombinant. Purified from E.coli strain carrying the NheI gene from Neisseria nucosa heidelbergensis.

Reaction Temperature: 37°C

Prototype: NheI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x NheI Buffer: 20 mM Tris-HCl (pH 7.4 at 25°C), 5 mM MgCl2, 50 mM KCl, 100 µg/ml bovine serum albumin.

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Ad-2 DNA in 1 hr in a total reaction volume of 20 µl.

Notes: 
- due to star activity it is not recommended to incubate over 4 hours or to use more than 100 units per reaction;
- salt concentration above 50 mM inhibits NheI activity.

Assay Conditions: 20 mM Tris-HCl (pH 7.4 at 25°C), 50 mM KCl, 5 mM MgCl2, 100 µg/ml bovine serum albumin and 1 µg of Ad-2 DNA. Incubation is at 37°C for 1 hr in a reaction volume of 20 µl.

Storage Buffer: 10 mM Tris-HCl (pH 7.5 at 25°C), 250 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% (v/v) Triton™X-100, 500 µg/ml bovine serum albumin and 50% (v/v) glycerol.

Storage Conditions: Store at –20°C.

Quality Control:
All preparations are assayed for contaminating endonuclease, 3'-exonuclease, and nonspecific single- and

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