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Mung Bean Nuclease

Marka:EURx

Ürün Ölçü Fiyat TL Miktar
Mung Bean Nuclease
E1190-01
2 000 u 72 EUR
+%20 KDV
3.542,40 TL
Mung Bean Nuclease
E1190-02
10 000 u 288 EUR
+%20 KDV
14.169,60 TL

Single-stranded specific DNA and RNA endonuclease.

Description:

  • Removes protruding ends in duplex DNA resulting in ligatable blunt ends (1).

  • Suitable for trimming single-stranded protruding ends of DNA or RNA without degrading the duplex structure (2).

  • Used for mapping of transcripts by analyzing the Mung Bean Nuclease-resistant RNA-DNA hybrids (3).

  • Digests hairpin structures during cDNA synthesis (4).

  • Will not cleave the strand opposite a nick in duplex DNA.

  • Requires Zn2+ ions for activity.

Unit Definition: One unit is the amount of enzyme required to produce 1 µg of acid-soluble material per minute at 37°C using denatured calf thymus DNA.

Storage Conditions: Store at -20°C.

Storage Buffer: 10 mM Tris-HCl (pH 7.5 at 22°C), 0.1 mM zinc acetate, and 50% (v/v) glycerol.

Reaction Buffer: 30 mM sodium acetate (pH 5.0 at 22°C), 30 mM NaCl, 1 mM ZnCl2.

Note: Also active in Low & Medium Buffers.

Assay Conditions: 30 mM sodium acetate (pH 5.0 at 22°C), 50 mM NaCl, 1 mM ZnCl2, 0.5 mg/ml denatured calf thymus DNA and 5% glycerol. Incubation is at 37°C for 10 min in a reaction volume of 1 ml.

Quality Control: All preparations are assayed for contaminating double-stranded DNase activity.

References:

  1. Ardelt, W., and Laskowski, M., Sr. (1971) Biochem. Biophys. Res. Commun. 44, No. 5, 1205-1211.

  2. Berk, A.J., and Sharp, P.A. (1977) Cell 12, 721-732.

  3. Berk, A.J., and Sharp, P.A. (1978) Proc. Natl. Acad. Sci. U.S.A. 75, 1274-1278.

  4. Goodman, H.M. and McDonald, R.J. (1979) Methods Enzymol. 68, 75-90.

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