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MboII, (MboII)

Marka:EURx

Ürün Ölçü Fiyat TL Miktar
MboII, (MboII)
E2284-01
250 u 57,6 EUR
+%20 KDV
2.833,92 TL
MboII, (MboII)
E2284-02
1 250 u 228,8 EUR
+%20 KDV
11.256,96 TL

MboII

(Moraxella bovis)

5’-G A A G A (N)8-3’
3’-C T T C T (N)7 -5’

Reaction Temperature: 37°C

Prototype: MboII

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x Low Buffer: 10 mM Tris-HCl, (pH 7.0 at 37°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 µg/ml bovine serum albumin.

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of unmethylated lambda DNA in 1 hr in a total reaction volume of 50 μl.

Assay Conditions: 10 mM Tris-HCl (pH 7.0 at 37°C), 10 mM MgCl2, 1 mM ditiothreitol, 100 µg/ml bovine serum albumin and 1μg of unmethylated lambda DNA. Incubation is at 37°C for 1 hr in a reaction volume of 50 µl.

To avoid star activity it is not recommended:
- to use more than 10 units per reaction;
- to incubate over 1 hr.

Storage Buffer: 10 mM potassium phosphate (pH 7.0), 50 mM KCl, 0.1 mM EDTA, 7 mM β-mercaptoethanol, 300 μg/ml bovine serum albumin and 50% (v/v) glycerol.

Storage Conditions: Store at –20°C.

Quality Control:

Non-specific Endonucleases: Incubation of 10 units of MboII with 1 µg of unmethylated lambda DNA at 37°C for 16 hrs (a 160-fold over-digestion) resulted in the same sharp characteristic banding pattern as the standard assay reaction, as determined by agarose gel electrophoresis.

3’-Exonuclease: 4, 8 and 16 units of MboII and 0.16 µg (1.23 pmol of 3’-ends) of lambda/TaqI fragments (3’-labeled with T4 DNA Polymerase and [³H]dGTP and [³H]dCTP), incubated for 1 hr at 37°C resulted in 0.05-slope of %-end label released per unit of enzyme. Reaction volume 10 µl.

5’-Exonuclease/5’-Phosphatase: Incubation of 4, 8 and 16 units of MboII with 0.03 µg (0.24 pmol of 5’-ends) of [5’-³²P] lambda/HaeIII fragments for 1 hr at 37°C resulted in 0.03-slope of %-end label released per unit of enzyme. Reaction volume 10 µl.

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