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Lambda Exonuclease

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Ürün Ölçü Fiyat TL Miktar
Lambda Exonuclease
E1180-01
1 000 u 72 EUR
+%20 KDV
3.542,40 TL
Lambda Exonuclease
E1180-02
5 000 u 288 EUR
+%20 KDV
14.169,60 TL

Double-stranded specific DNase produced by Escherichia coli upon lambda bacteriophage infection that digests one DNA strand starting from 5’ terminus.

Description:

  • Double-stranded specific DNase that requires the presence of a 5'-phosphate group for activity.

  • PCR products prepared using one PCR primer phosphorylated at the 5'-end and the other primer unphosphorylated can be treated with lambda exonuclease to yield a single-stranded DNA.That helps to obtain a clean readable sequence without the extraneous bands which are often present when PCR products are sequenced directly.

  • DNA digestion with lambda exonuclease is especially helpful when sequencing PCR products with a high GC content.

Unit Definition: One unit produces 10 nmoles of acid-soluble deoxribonucleotide from double-stranded DNA in 30 min at 37°C.

Storage Conditions: Store at –20°C.

For unambiguous PCR Sequencing:

  1. digest purified DNA with Recombinant Lambda Exonuclease

  2. heat to inactivate enzyme

  3. ready for DNA sequencing

Assay Conditions: 67 mM glycine-KOH (pH 9.4), 50 µg/ml bovine serum albumin, 2.5 mM MgCl2, 20 µg/ml sonicated [³H]-labeled lambda DNA and lambda exonuclease in 50 µl for 30 min at 37°C.

Quality Control: All preparations are assayed for contaminating nonspecific endodeoxyribonuclease and 3' exodeoxyribonuclease activities. Tested for the presence of linear DNA.

References:

  1. Little, John W. (1981)Gene Amplification and Analysis 2, 135-145.

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