Gp 32 Protein(single-stranded binding protein of T4 bacteriophage)

Single-stranded DNA Binding Protein of T4 bacteriophage

Source: E.coli cells infected by T4 bacteriophage.

Description:

• Single-stranded specific DNA binding protein (1).
• Helix destabilizing protein (1).
• Reduces formation of secondary DNA structures.
• Prevents degradation of ssDNA by nucleases.
• Ultrapure recombinant protein.
• Prevents inhibition of PCR by template DNA contaminants (2).
• Improves the efficiency of DNA amplification by Taq Polymerase (3,4,5,6).
• Replaces Hot Start method during assembling PCR reaction (4,7).
• Stabilizes single-stranded regions of DNA for site-specific mutagenesis.
• Aids completion of restriction enzyme digestion.
• Improves the efficiency of DNA synthesis by T4 DNA Polymerase (8).
• Enhances the fidelity of modified T4 Polymerase (8).

Storage Conditions: Store at –20°C.

Storage Buffer: 20 mM Tris-HCl (pH 7.8 at 22°C), 300 mM NaCl, 5 mM β-mercaptoethanol, 0.05% Igepal, 0.2 mM EDTA and 50% (v/v) glycerol.

Quality Control: All preparations are assayed for contaminating endonuclease, 3'- and 5'-exonuclease activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.

References:

1. Greipel, J. Urbanke, C. and Maass, G. (1989) in: Saenger, W., Heinemann, U. (Eds.) pp. 61-86.
2. Kreader, C.A. (1996) Applied Environ. Micro. 62, 1102-1106.
3. D�browski, S., Olszewski, M., Pi�tek, R. and Kur, J. (2002) Protein Expr. Purif. 26, 131-138.
4. D�browski, S. and Kur, J. (1999) Protein Expr. Purif. 16, 96-102.
5. Rapley, Mol. Biotech. 2 (1994) 295-298.
6. Schwarz, K., Hansen-Hagge, T. and Bartram, C. (1989) Nucleic Acids Res. 18, 1079.
7. Barski, P., Piechowicz, L., Galinski, J. and Kur, J. (1996) Mol. Cell Probes 10, 471-475.
8. Sandhu, D.K. and Keohavong, P. (1994) Gene 144, 53-58.