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GeneMATRIX DNA/RNA/Protein Purification Kit
GeneMATRIX DNA/RNA/Protein Purification Kit is designed for rapid purification of genomic DNA, total RNA and total protein simultaneously from a single biological sample from a wide variety of bacterial physiological groups, animal tissue, plant and from a wide variety of yeast strains. Samples are first disrupted, homogenized and lysed in the presence of lysis and denaturing buffers, which inactivates DNases and RNases as well as proteases. In the next stage, DNA binding spin columns reduce viscosity of the lysate and bind DNA fragments. Then sample is applied to a RNA binding spin column where all RNA molecules are adsorbed to the matrix and contaminants are efficiently washed away. High-quality RNA is then eluted in RNase-free water. Typical yields are up to 100 µg total RNA longer than 200 bases. Proteins are precipitated from the flow-through of RNA binding spin column and are pelleted by centrifugation. The kit includes buffer PLB which is compatible with SDS-PAGE for dissolving the protein pellet. This kit is ideal for researchers who are interested in studying the genome, proteome and transcriptome of a single sample.