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GeneMATRIX Human Blood RNA Purification Kit is designed for rapid purification of total RNA from fresh human blood. Purified RNA is free of contaminants, such as: DNA, proteins, lipids, dyes, detergents, organic inhibitors of enzymatic reactions, buffers, salts, divalent cations, among others. In the first step the red blood cells are lysed and the remaining leukocytes are spun. Then leukocytes are lysed in the presence of denaturing buffer, which inactivates cellular RNases. In the next stage, homogenization spin-columns shear genomic DNA, reducing viscosity of the lysate and eliminating DNA fragments. Then sample is applied to a binding spin-column where all RNA molecules are adsorbed to the matrix and contaminants are efficiently washed away. High-quality RNA is then eluted in RNase-free water. With a good performance we get a total RNA longer than 200 bases. However, it is possible to purify RNA molecules smaller than 200 bases, with gradually decreasing efficiency. Isolated RNA is ready for downstream applications without the need for ethanol precipitation.
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