Dünyada bir ilk 1 ay boyunca oda sıcaklığında özelliğini yitirmeden muhafaza edilebilir.
HOT FIREPol® DNA Polymerase is a chemically modified FIREPol® DNA Polymerase.
At ambient temperatures it is inactive, having no polymerization activity.
HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C.
This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→5’ exonuclease activity.
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