NGS qPCR Library Quantification Kit with EvaGreen®

NGS qPCR Library Quantification Kit with EvaGreen® for Illumina sequencing platforms is a ready-to-use kit for quantifying amplifiable molecules in DNA library. Since qPCR only measures molecules that have sequencing adapter it prevents under or overestimation of library concentration, which may lead to poor results in sequencing reaction. Kit comprises all the components necessary to perform library quantification with qPCR: 5x HOT FIREPol® EvaGreen® Library Quantification Mix, 50x Illumina Primer Premix and DNA Standards (six 450bp in 10-fold serial dilutions).

Applications:

                Suited for quantification of libraries

                Broad range of concentrations

                Suitable for wide range of GC concentrations

Suitable qPCR Platforms: 5x HOT FIREPol® EvaGreen® Library Quantification Mix is compatible with all qPCR platforms regardless of ROX requirements.