Perfect™ 100-1000bp DNA Ladder

DNA ladder with 100 bp increments for sizing small-to-medium DNA fragments.

Description:

• Ideal for sizing linear double-stranded DNA fragments from 100 to 1000 bp.
• Contains 10 bands with fragments of the following sizes: 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000 bp.
• Band at 500 bp is three times brighter for easy reference on agarose gels.
• Can be 5'-end labeled with radioisotopes and T4 Polynucleotide Kinase for visualization by autoradiography after a dephosphorylation step.

Storage Conditions: Store at -20°C.

Storage Buffer: 10 mM Tris-HCl (pH 8.0 at 22°C), 1 mM EDTA, dye.

Loading: The recommended amount of size marker to load on a gel is 2-5 µl per lane depending on a gel type and size of well.

Concentration: The Perfect™100-1000 bp DNA Ladder is supplied at 47.9 µg/ml in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA.