Rec A Protein (E.coli)

Bacterial recombination protein that enhances interaction and exchange of homologous DNA strands.

Description:

• A 37,8 kDa protein encoded by the recA gene of E. coli.
• Regulates repair mechanisms and repairs DNA (1, 2).
• Involved in DNA renaturation and rare cleavage of genomic DNA.
• Binds cooperatively and stechiometrically to single-stranded DNA.
• Ultrapure recombinant protein.
• In presence of ATP-g-S, locates and pairs a single-stranded DNA sequence to its homologous double-stranded DNA.
• Cleaves large fragments of DNA (RARE-Rec A Assisted Restriction Endonuclease) (3).
• Enhances contrast of electron micrography when DNA is coated with Rec A Protein (4).

  Storage Buffer: 20 mM Tris-HCl (pH 7.5 at 22°C), 1 mM dithiothreitol, 0.1 mM EDTA and 50% (v/v) glycerol.

  Storage Conditions: Store at -20°C.

  Quality Control: All preparations are assayed for endonuclease and nonspecific single- and double-stranded DNase activities. Typically preparations are greater than 95% pure as judged by SDS polyacrylamide gel electrophoresis.

  References:

  1. Clark, A.J. and Margulies, A.D. (1965) Proc. Natl. Acad. Sci. U.S.A. 53, 451-459.
  2. Radding, C.M. (1978) Annu. Rev. Biochem. 47, 847-880.
  3. Koob, M., Burkiewicz, A., Kur, J. and Szybalski, W. (1992) Nucleic Acids Res. 20, 5831-5836.
  4. Krasnow, M. A. et al. (1983) Nature 304, 559. contrast of electron micrography when DNA is coated with Rec A Protein (4).